Human Pluripotent Stem Cell Workshop

Embryonic stem cells (Image/courtesy of Qi-Long Ying)
Embryonic stem cells (Image/courtesy of Qi-Long Ying)

The USC Stem Cell Core will be hosting an introductory three-day course in pluripotent stem cell techniques aimed at researchers who are interested in performing research with human embryonic stem cells (hESC).

This course will be held on March 17–19 at the Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC.

Topics include:

  • Preparation of MEF feeder plates
  • Preparation of Matrigel coated plates
  • Feeder Free culture (mTESR1, StemPro and Matrigel/MEF-CM)
  • Enzymatic passaging onto MEF feeders
  • Manual passaging onto MEF feeders
  • Cryopreservation of human pluripotent stem cells (hPSC) by slow freezing
  • Thawing hPSC
  • Quality control testing hPSC cultures

This course is a must for researchers growing hESC.

Course fees: $500

Contact: Sunny D. Bhalla, 323-422-7834,,