Metal detection has helped mining companies strike gold and airport security identify passengers who are a potential threat. Now USC Dornsife scientists have pushed its use into another realm: studying cancer.
By imaging metal-tagged antibodies on biopsies from a patient with metastatic prostate cancer, researchers at the USC Michelson Center for Convergent Bioscience Bridge Institute have created highly detailed, digital facsimiles of cancer cells that can travel through the body. The metal tags enable scientists to identify and characterize the cancer cells in a blood sample after it is placed on a slide.
“That is exactly what is happening when the TSA swipes your hands,” said USC Dornsife’s Peter Kuhn, professor of biological sciences and associate director of the Bridge Institute. “They are looking for metals, which are really easy to identify.”
The USC Dornsife-led study, published in January by Convergent Science Physical Oncology, established the proof of concept for the metal-detection technique, which allows scientists to see circulating and disseminated tumor cells at a molecular level in a way not possible before. Creating such highly detailed copies of tumors may help researchers develop more precise treatment plans for individual patients.
“We are trying to understand how cancer actually moves from the initial location to other places in the body and can settle there,” said Kuhn, professor of medicine at Keck School of Medicine of USC and biomedical engineering, and aerospace and mechanical engineering at USC Viterbi School of Engineering. Kuhn’s professorships at USC Dornsife, USC Viterbi and Keck School of Medicine are all tied to the USC Michelson Center, a hub for convergent bioscience at the university.
Through his work, Kuhn, whose research bridges multiple fields, aims to shed new light on how cancer spreads through the body and evolves over time. Such discoveries have already led to better personalized care for patients, which tailors the treatment to the individuals as much as to their specific form of cancer.
Mapping cancer for precision medicine
The study examined whether scientists could achieve a better blueprint for the spread of the tumor, which is the most difficult phase of cancer. Cancer spreads via rare circulating and disseminated tumor cells that break away from their original source, such as tumors in the breast or the prostate, and travel through the body. These rogue tumor cells spread into organs, such as the liver or lungs, or into the bones, where they metastasize undetected, making effective treatment very challenging.
Until now, researchers have relied on fluorescence microscopy — staining cells with a fluorescent labeled antibody — and then examining them with microscopes. Fluorescence microscopy is useful, but its routine use is limited in the number of colors available in a single experiment.
With the Fluidigm Hyperion Imaging System to monitor the biology of the cancer cells and understand how the cancer changes, scientists could see protein biomarkers that may determine how a tumor cell would respond to a drug therapy or why it would fail to respond, how it could spread and how it might affect the patient’s immune system response.
The new approach of using metal-tagged antibodies and a laser ablation system, coupled with a mass spectrometer, gives scientists the ability to track 35 different metal labels simultaneously.
As a result, it provides 35 distinct views of the cancer cell’s biology, Kuhn said.
“Oftentimes, we sequence the cancer’s genetic code, and that’s great because the only way to build something like a building or a machine is with a blueprint. But not every blueprint ends up being built to specification or even perform as expected,” Kuhn said. “For a closer perspective and for purposes of improving the precision of medical treatment, you have to move in, from genome to proteome to cell.”
Zooming in on cancer
Looking at cancer is like seeing a painting at different distances. From afar, it is one of Monet’s water lily paintings. But up close, nearly touching the canvas, one can see the individual points of paint in varying colors and shapes that constitute every object within the painting.
When it comes to studying circulating and disseminated cancer cells, scientists need to see those points and be able to zoom in and out to fully grasp how they behave and spread. They especially want to capture this picture just as they are determining the course of treatment, which the metal-tracing technique enables them to do within a liquid biopsy.
Metals characterize cancer
Researchers at the University of Zurich established the potential for using metals to characterize cancer in 2013.
“Bernd Bodenmiller did some elegant work on how to use metals attached to an antibody. We expanded on that by using his approach with the liquid biopsy that we had previously developed. We simply add the antibody cocktail, wait a while for binding and then wash off the excess and see what sticks — like tie dye,” Kuhn explained. “Then, you use a laser to atomize the sample and a mass spectrometer to look for each of the metals.”
Thanks to proof-of-concept studies like this, the technique is now an official product of Fluidigm and is available for researchers worldwide.
“This is really just the beginning,” Kuhn said. “You’ll see hundreds of studies now using this technique.”
USC Dornsife’s James Hicks, professor (research) of biological sciences at USC Michelson Center, was also a study co-author.
About the study
USC co-authors for the study included Erik Gerdtsson and Anders Carlsson of the Bridge Institute, as well as Akil Merchant and Mohan Singh of the USC Norris Comprehensive Cancer Center at the Keck School of Medicine.
Other USC co-authors were Anna Sandstrom Gerdtsson, Paymaneh Malihi, Rafael Nevarez, Anand Kolatkar, Carmen Ruiz Velasco and Sophie Wix. Additional co-authors were Jana-Aletta Thiele at Charles University in Prague; and Amado Zurita and Christopher Logothetis at the University of Texas MD Anderson Cancer Center.
The work was funded by grants from the Breast Cancer Research Foundation, the National Cancer Institute and Leidos Biomedical Research, Prostate Cancer Foundation, Vassiliadis Research Fellowship, Polak Research Fellowship, the Vicky Joseph Research Fellowship and the Charles University Research Fund.
USC is an early access partner of Fluidigm, which provided reagents and expertise to support the study.
USC Michelson Center
James Hicks and Peter Kuhn, whose work was highlighted as part of former Vice President Joe Biden’s Cancer Moonshot initiative, are among an estimated 20 scientists and engineers based at the USC Michelson Center from USC Dornsife, USC Viterbi School of Engineering and Keck School of Medicine of USC. By collaborating across multiple disciplines, they are working to solve some of the greatest intractable problems of the 21st century in biomedical science, including cancer.
Working side by side, USC Michelson Center’s researchers aim to hasten the development of new drug therapies, high-tech diagnostics and biomedical devices from the bench to the bedside.